Oligonucleotide probes for analyzing 40 types of HPV were synthesized, and
DNA chips were produced by using the oligonucleotide probes. The
synthesis of the oligonucleotide probes is based on clones of L1 and
E6/E7 genes of 35 types of HPV obtained from cervical cell specimens from
4,898 Korean adult women and tissue specimens from 68 cervical cancer
cases in addition to information based on American and European cases.
The DNA chips can analyze the 40 types of HPV found in cervical, diagnose
complex infection by at least one type of HPV, and have excellent
diagnostic sensitivity and specificity on HPV genetic type up to 100% and
reproducibility. Also, the DAN chips are superior to conventional
analytic method, and very economical, since they can analyze numerous
specimens in shortest time. Accordingly, the DNA chips are useful for
predicting cervical cancer and precancerous lesion.