The present invention relates to mutant .DELTA.8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 .omega.-6, EDA] to dihomo-.gamma.-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 .omega.-3, ETrA] to eicosatetraenoic acid [20:3 .omega.-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding .DELTA.8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant .DELTA.8 desaturases in plants and oleaginous yeast are disclosed.

 
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