Techniques for analyzing gene expression levels are provided. In one aspect
of the invention, the technique provides a method for determining a
concentration level of a target nucleic acid, the target nucleic acid
comprising at least one target oligonucleotide. The method determines (i)
a measure of affinity value of the target oligonucleotide with a probe
oligonucleotide; and (ii) a hybridization intensity value for the target
oligonucleotide and the probe oligonucleotide at a probe spot. The measure
of affinity value and the hybridization intensity value are used to
determine the concentration level of the target nucleic acid.
