Antibodies to two new epitopes on the HCV envelope proteins were identified
which allow routine detection of native HCV envelope antigens, in tissue
or cells derived from the host. The new epitopes are: the E1 region aa
307-326 and the N-terminal hyper variable region of E2 aa 395-415.
Surprisingly, we characterised an antibody that reacts with various
sequences of the hypervariable domain of E2. Specific monoclonal
antibodies directed against these epitopes and allowing routine detection
of viral antigen are described.
Os antibodies a dois epitopes novos nas proteínas do envelope de HCV foram identificados que permitem a deteção rotineira de antígenos nativos do envelope de HCV, no tecido ou nas pilhas derivado do anfitrião. Os epitopes novos são: E1 a região aa 307-326 e a região variável hyper do N-terminal do E2 aa 395-415. Surprisingly, nós caracterizamos um antibody que reagisse com as várias seqüências do domínio hypervariable do E2. Os antibodies monoclonal específicos dirigidos de encontro a estes epitopes e a permitir a deteção rotineira do antígeno viral são descritos.
