Reagent for the isolation of RNA

   
   

The present invention provides RNA extraction reagents, methods and kits that are especially useful for extracting RNA. The reagents, methods and kits of the present invention are especially useful for extracting RNA, for example, cytoplasmic RNA, from difficult materials, from plants, especially, difficult plant tissues, such as those containing phenolics, tannins, polysaccharides (such as starch) and resins. Comparative high yields are obtainable according to the present invention when compared to conventional reagents and methods. The RNA preparations obtained in accordance with the present invention are also of high quality as demonstrated by superior A.sub.260/280 results and by gel electrophoresis.

De onderhavige uitvinding verstrekt de extractiereagentia, methodes en uitrustingen van RNA die vooral nuttig zijn om RNA te halen. De reagentia, de methodes en de uitrustingen van de onderhavige uitvinding zijn vooral nuttig om RNA, bijvoorbeeld, cytoplasmic RNA, uit moeilijke materialen, van installaties, vooral, moeilijke installatieweefsels, zoals die te halen die phenolics, tannine, polysacchariden (zoals zetmeel) bevatten en harsen. De vergelijkende hoge opbrengsten zijn verkrijgbaar volgens de onderhavige uitvinding wanneer vergeleken bij conventionele reagentia en methodes. De voorbereidingen van RNA die overeenkomstig de onderhavige uitvinding worden verkregen zijn ook van uitstekende kwaliteit zoals die door superieure resultaten A.sub.260/280 en door gelelektroforese worden aangetoond.

 
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