The invention encompasses compositions and methods which utilize a cell line
comprising a stably integrated recombinant nucleic acid construct comprising: a
reporter gene operably linked to a recognition sequence for a sequence-specific
DNA-binding protein; and a stably integrated recombinant nucleic acid construct
comprising a sequence encoding a fusion protein, the fusion protein comprising
a sequence-specific DNA binding domain, wherein the DNA binding domain specifically
binds the recognition sequence, and a conditionally active transactivation domain
of CHOP, wherein binding of the fusion protein to the recognition sequence results
in transactivation of the reporter gene when the transactivation domain fused to
the DNA binding domain is activated.
