A method of emulating with a cyanide-free lyse solution, the measurement of hemoglobin
in whole blood using a cyanide-containing lyse solution. Such an illustrative method
includes: a) combining a predetermined amount of the whole blood sample with a
predetermined amount of a cyanide-free lyse solution to form a mixture, b) developing
the mixture to a molar absorbtivity () in the range of about 12.4 to 12.6
mM-1cm-1, and c) measuring a level of light absorbance of
the mixture at a wavelength of about 540 nm. In one preferred illustrative embodiment,
the cyanide-free lyse solution includes: a quaternary ammonium salt surfactant,
an anionic surfactant, a hemoglobin binding agent selected from the group consisting
of imidazole or hydroxylamine, and an aqueous medium. In one preferred embodiment,
the molar absorbtivity is developed to a value of about 12.5 mM-1cm-1.
That is to say to a level that is substantially identical to that of the prior
art standard, cyanomethemoglobin. By achieving this level of molar absorbtivity,
the final calculated results do not have to be corrected for the different molar
absorbtivity exhibited by imidazolemethemoglobin or hydroxylaminemethemoglobin.
