A method of promoting tissue adhesion to reinforce tissue apposition that may include collecting a first tissue portion into a first fold, placing a first tissue apposition means through the first fold, collecting a second tissue portion into a second fold, placing a second tissue apposition means through the second fold, tightening the first and second tissue apposition means to approximate the first and second tissue folds, and placing a fixation agent at least partially between the first and second tissue folds. In this illustrative embodiment, the method promotes tissue adhesion between one or more portions of tissue, wherein the tissue adhesion may reinforce a tissue apposition.

 
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