The present invention relates to a brightness enhancement film and a backlight module having the brightness enhancement film. The brightness enhancement film includes first micro-reflectors and second micro-reflectors. The cross-section of each of the first and second micro-reflectors is a triangle having two same or different base angles and a wavy crest line. The widths of the first and second micro-reflectors vary along with the valleys and peaks to broaden or to narrow with respect to two sides thereof such that the neighboring lines between the first and second micro-reflectors are wavy. The backlight module is coupled with the brightness enhancement film. This enhances brightness, illuminates evenly, and avoids a moire effect.